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首页> 外文OA文献 >Sequential Partially Overlapping Gene Arrangement in the Tricistronic S1 Genome Segments of Avian Reovirus and Nelson Bay Reovirus: Implications for Translation Initiation
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Sequential Partially Overlapping Gene Arrangement in the Tricistronic S1 Genome Segments of Avian Reovirus and Nelson Bay Reovirus: Implications for Translation Initiation

机译：禽呼肠孤病毒和尼尔森湾呼肠孤病毒的Tricistronic S1基因组片段中的顺序部分重叠的基因安排：对翻译开始的影响。

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Previous studies of the avian reovirus strain S1133 (ARV-S1133) S1 genome segment revealed that the open reading frame (ORF) encoding the ςC viral cell attachment protein initiates over 600 nucleotides distal from the 5′ end of the S1 mRNA and is preceded by two predicted small nonoverlapping ORFs. To more clearly define the translational properties of this unusual polycistronic RNA, we pursued a comparative analysis of the S1 genome segment of the related Nelson Bay reovirus (NBV). Sequence analysis indicated that the 3′-proximal ORF present on the NBV S1 genome segment also encodes a ςC homolog, as evidenced by the presence of an extended N-terminal heptad repeat characteristic of the coiled-coil region common to the cell attachment proteins of reoviruses. Most importantly, the NBV S1 genome segment contains two conserved ORFs upstream of the ςC coding region that are extended relative to the predicted ORFs of ARV-S1133 and are arranged in a sequential, partially overlapping fashion. Sequence analysis of the S1 genome segments of two additional strains of ARV indicated a similar overlapping tricistronic gene arrangement as predicted for the NBV S1 genome segment. Expression analysis of the ARV S1 genome segment indicated that all three ORFs are functional in vitro and in virus-infected cells. In addition to the previously described p10 and ςC gene products, the S1 genome segment encodes from the central ORF a 17-kDa basic protein (p17) of no known function. Optimizing the translation start site of the ARV p10 ORF lead to an approximately 15-fold increase in p10 expression with little or no effect on translation of the downstream ςC ORF. These results suggest that translation initiation complexes can bypass over 600 nucleotides and two functional overlapping upstream ORFs in order to access the distal ςC start site.
机译：先前对禽呼肠孤病毒S1133（ARV-S1133）S1基因组片段的研究表明，编码ςC病毒细胞附着蛋白的开放阅读框（ORF）起始于S1 mRNA 5'末端的600多个核苷酸。两个预测的小型非重叠ORF。为了更清楚地定义这种不寻常的多顺反子RNA的翻译特性，我们进行了相关纳尔逊湾呼肠孤病毒（NBV）S1基因组片段的比较分析。序列分析表明，存在于NBV S1基因组片段上的3'-近端ORF也编码ςC同源物，这是通过存在与螺旋体细胞粘附蛋白相同的卷曲螺旋区所特有的扩展的N末端七肽重复序列所证实的。呼肠孤病毒。最重要的是，NBV S1基因组片段在ςC编码区上游包含两个保守的ORF，相对于ARV-S1133的预测ORF而言它们是延伸的，并以顺序，部分重叠的方式排列。对另外两个抗逆转录病毒株的S1基因组片段的序列分析表明，与NBV S1基因组片段所预测的相似，三顺反子基因的排列方式相似。 ARV S1基因组片段的表达分析表明，所有三个ORF在体外和在病毒感染的细胞中均具有功能。除了先前描述的p10和ςC基因产物外，S1基因组片段还从中央ORF编码一个未知功能的17 kDa碱性蛋白（p17）。优化ARV p10 ORF的翻译起始位点可导致p10表达增加约15倍，而对下游ςCORF的翻译几乎没有影响。这些结果表明，翻译起始复合物可以绕过600个核苷酸和两个功能重叠的上游ORF，以访问远端ςC起始位点。

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